Pengaruh frekuensi konsumsi kopi robusta (coffea canephora) terhadap gambaran histopatologi dan biokimia ginjal pada tikus putih jantan galur wistar
DOI:
https://doi.org/10.34305/jikbh.v15i02.1214Keywords:
Kopi, Robusta, Frekuensi, Ginjal , TikusAbstract
Latar Belakang: Kopi merupakan minuman yang umum dikonsumsi dan memiliki kemampuan sebagai antioksidan yang dapat memberikan manfaat terhadap kesehatan ginjal. Tujuan dari penelitian untuk melihat efek frekuensi konsumsi kopi robusta terhadap gambaran histopatologis dan biokimia organ ginjal meliputi kadar ureum dan kreatinin pada model hewan uji tikus galur wistar.
Metode: Penelitian ini bersifat eksperimental dengan desain Posttest Only Group Control. Tikus dibagi secara acak menjadi 4 kelompok perlakuan (masing-masing terdiri dari 5 ekor). Perlakuan per oral ekstrak kopi diberikan selama 30 hari untuk dievaluasi parameter histopatologis dan fungsi ginjal.
Hasil: Hasil penelitian menunjukkan kadar ureum dan kreatinin serum tidak ada perbedaan yang signifikan pada setiap peningkatan pemberian dosis kopi robusta setelah 30 hari masa penelitian. Skoring histopatologis ginjal mencit tidak menunjukan perbedaan yang signifikan pada kelompok yang diberikan kopi robusta terhadap kelompok kontrol (p value < 0,005).
Kesimpulan: Penelitian ini menyimpulkan bahwa konsumsi kopi Robusta tidak memberikan efek negatif terhadap fungsi dan struktur histopatologis ginjal mencit. Temuan tersebut melengkapi pengetahuan tentang manfaat dari konsumsi kopi yang tidak berdampak pada kesehatan ginjal.
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References
Adeyomoye, O. I., Akintayo, C. O., Omotuyi, K. P., & Adewumi, A. N. (2022). The biological roles of urea: A review of preclinical studies. Indian Journal of Nephrology, 32(6), 539–545. https://doi.org/https://doi.org/10.4103/ijn.IJN
Agi, Y. A., & Titrawani, T. (2021). Kidney Histology of Wistar Rats (Rattus norvegicus Berkenhout 1769) Due to White Coffee. Jurnal Biologi Unand, 9(2), 60–67. https://doi.org/https://doi.org/10.25077/jbioua.9.2.60-67.2021
Al-Ghifari, A. W. F., Sari, G. M., Tinduh, D., Purwanto, B., & Yuliawati, T. H. (2023). Protective Effect of Toraja Robusta Coffee (Coffee cane-Nora) against Muscle Damage Due to Exercise on Balb/C Mice. Tropical Journal of Natural Product Research, 7(12). https://doi.org/https://doi.org/10.26538/tjnpr/v7i12.33
Amini, N., Sadeghi, A., & Afif, A. H. (2020). The Effect of Eight-week Caffeine Supplementation and High-Intensity Interval Training on the Serum Urea and Creatinine Levels and Morphological Changes of Glomerular Unit in Diabetic Rats. Journal of Kerman University of Medical Sciences, 27(6). https://doi.org/https://doi.org/10.22062/JKMU.2020.91527
Amini, T., & Hashemi, P. (2018). Preconcentration and GC–MS determination of caffeine in tea and coffee using homogeneous liquid–liquid microextraction based on solvents volume ratio alteration. Journal of Chromatography B, 1092, 252–257. https://doi.org/https://doi.org/10.1016/j.jchromb.2018.06.020
Erskine, E., Gültekin Subaşı, B., Vahapoglu, B., & Capanoglu, E. (2022). Coffee phenolics and their interaction with other food phenolics: Antagonistic and synergistic effects. ACS Omega, 7(2), 1595–1601. https://doi.org/https://doi.org/10.1021/acsomega.1c06085
Faria, W. C. S., de Oliveira, M. G., da Conceição, E. C., Silva, V. B., Veggi, N., Converti, A., de Barros, W. M., da Silva, M. F., & Bragagnolo, N. (2020). Antioxidant efficacy and in silico toxicity prediction of free and spray-dried extracts of green Arabica and Robusta coffee fruits and their application in edible oil. Food Hydrocolloids, 108, 106004. https://doi.org/https://doi.org/10.1016/j.foodhyd.2020.106004
Heath, J. C., Abdelmageed, Y., Braden, T. D., & Goyal, H. O. (2012). The effects of chronic ingestion of mercuric chloride on fertility and testosterone levels in male Sprague Dawley rats. BioMed Research International, 2012(1), 815186. https://doi.org/https://doi.org/10.1155/2012/815186
Heryanto, M. L., & Moonti, M. A. (2023). Efektifitas pemberian smoothies pisang ambon dan jambu biji merah terhadap kenaikan hemoglobin (Hb) pada remaja puteri di Sekolah Tinggi Ilmu Kesehatan Kuningan. Jurnal Ilmu Kesehatan Bhakti Husada: Health Sciences Journal, 14(02), 242–249. https://doi.org/https://doi.org/10.34305/jikbh.v14i02.852
Hwang, S. J., Kim, Y.-W., Park, Y., Lee, H.-J., & Kim, K.-W. (2014). Anti-inflammatory effects of chlorogenic acid in lipopolysaccharide-stimulated Raw 264.7 cells. Inflammation Research, 63, 81–90. https://doi.org/https://doi.org/10.1007/s0001101306744
Krieger, D. R., Kalman, D. S., Feldman, S., Arnillas, L., Goldberg, D., Gisbert, O., & Nader, S. (2016). The safety, pharmacokinetics, and nervous system effects of two natural sources of caffeine in healthy adult males. Clinical and Translational Science, 9(5), 246–251. https://doi.org/https://doi.org/10.1111/cts.12403
Leta, B., Kenenisa, C., Wondimnew, T., & Sime, T. (2021). Evaluation of Renoprotective Effects of Our Locally Grown Green Coffee Beans against Cisplatin‐Induced Nephrotoxicity in Swiss Albino Mice. International Journal of Nephrology, 2021(1), 2805068. https://doi.org/https://doi.org/10.1155/2021/2805068
Liang, N., & Kitts, D. D. (2014). Antioxidant property of coffee components: assessment of methods that define mechanisms of action. Molecules, 19(11), 19180–19208. https://doi.org/https://doi.org/10.3390/molecules191119180
Ontawong, A., Duangjai, A., Vaddhanaphuti, C. S., Amornlerdpison, D., Pengnet, S., & Kamkaew, N. (2023). Chlorogenic acid rich in coffee pulp extract suppresses inflammatory status by inhibiting the p38, MAPK, and NF-κB pathways. Heliyon, 9(3). https://doi.org/https://doi.org/10.1016/j.heliyon.2023.e13917
Rojas-González, A., Figueroa-Hernández, C. Y., González-Rios, O., Suárez-Quiroz, M. L., González-Amaro, R. M., Hernández-Estrada, Z. J., & Rayas-Duarte, P. (2022). Coffee chlorogenic acids incorporation for bioactivity enhancement of foods: A review. Molecules, 27(11), 3400. https://doi.org/https://doi.org/10.3390/molecules27113400
Tandi, J., Marsella, M., Suarsana, I. M. A., Dewi, N. P., Magfirah, M., Handayani, T. W., & Kanan, M. (2023). The Effectiveness of Ethanol Extract of Robusta Coffee Seeds on Blood Glucose, Urea, and Creatinine Levels of Male White Rats Induced by Streptozotocin. Jurnal Farmasi Galenika (Galenika Journal of Pharmacy)(e-Journal), 9(2), 284–292. https://doi.org/https://doi.org/10.22487/j24428744.2023.v9.i2.16352
Tiera, H., & Umbas, R. (2013). Pemeriksaan Rapid Urinary Bladder Cancer Antigen untuk Deteksi Karsinoma Sel Transisional Buli pada Populasi Indonesia (Penelitian Awal). Indonesian Journal of Cancer, 7(2), 41–46. https://doi.org/10.33371/ijoc.v7i2.279
Wiliyanarti, P. F., Atrasina, J., & Maulidiyanti, E. T. S. (2021). Studi Pemeriksaan Sedimen Urine berdasarkan Karakteristik Pada Penikmat Kopi di Asrama Kiwal Brawijaya Surabaya. The Journal Of Muhammadiyah Medical Laboratory Technologist, 4(2), 157–169. https://doi.org/https://doi.org/10.30651/jmlt.v4i2.11135
Wisudawati, F. D., Hidajati, N., Fikri, F., Santoso, K. P., Saputro, A. L., & Purnama, M. T. E. (2023). The Potentials of Robusta Coffee Seed Extract as Antioxidant on Kidney Histopathology in Mice Exposed to Monosodium Glutamate: Potensi Pemberian Ekstrak Biji Kopi Robusta sebagai Antioksidan Terhadap Gambaran Histopatologi Ginjal Mencit yang Dipapar Monos. Veterinary Biomedical and Clinical Journal, 5(1), 19–24. https://doi.org/https://doi.org/10.21776/ub.vetbioclinj.2023.005.01.3
Wright, T. L. (2016). Policy Brief for the International Coffee Organization (ICO) on Behalf of the International Labour Organization (ILO) Regarding Human Rights Violations on Coffee Farms in Guatemala. Political Science Undergraduate Review, 2(1), 85–93. https://doi.org/https://doi.org/10.29173/psur68
Yosa, D., & Wibowo, A. (2023). Correlation between blood urea and creatinine level in patients with diabetes melitus at the ministry of marine and fishery clinic, Indonesia. The International Conference on Public Health Proceeding, 4(02), 576–584. https://doi.org/https://doi.org/10.26911/the6thicph.05.09
Yuan, S., & Larsson, S. C. (2022). Coffee and caffeine consumption and risk of kidney stones: a mendelian randomization study. American Journal of Kidney Diseases, 79(1), 9–14. https://doi.org/https://doi.org/10.1053/j.ajkd.2021.04.018
Zhu, J., Smith‐Warner, S. A., Yu, D., Zhang, X., Blot, W. J., Xiang, Y., Sinha, R., Park, Y., Tsugane, S., & White, E. (2021). Associations of coffee and tea consumption with lung cancer risk. International Journal of Cancer, 148(10), 2457–2470. https://doi.org/https://doi.org/10.1002/ijc.33445
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